What is “Methylation”?
Methylation is a biochemical process with numerous reactions that occur in every cell of our bodies in which a methyl group (CH3) is added to a substrate such as hormones, DNA, neurotransmitters, and immune cells.
This simple biochemical reaction is involved in the production of genetic material, regulation of gene expression, as well as metabolic processes, and can have a positive or negative impact on nearly every system in our bodies!
Why is Methylation Important?
Methylation is used throughout the body for some of these functions:
Impaired function of the methylation cycle can lead to a wide range of health challenges and conditions, including:
● Alzheimer’s disease
● Autoimmune disorders
● Birth defects
● Cardiovascular disease
● Infertility and Miscarriage
● Parkinson’s disease
● Thyroid disease
● Chronic viral infections
What Can Impair Methylation?
Many factors impact the methylation cycle, such as genetic mutations called SNPs (single nucleotide polymorphisms), diet, lifestyle, and environment. The most commonly looked at SNP that influences methylation activity is MTHFR.
It starts with MTHFR: We need a critical B vitamin, 5-MTHF (a.k.a, active folate or methylfolate), to start the methylation cycle. MTHFR (methylenetetrahydrofolate reductase) is an enzyme that converts the inactive form of folate to methylfolate to be used in this cycle. There are MTHFR gene variants or SNPs that reduce the function of this enzyme, and some research estimates that up to 40% of people in the U.S. have a MTHFR genetic mutation. Here is a general flow of how methylation flows from a systemic process point of view:
Graphic from: Luciano-Mateo F, Hernández-Aguilera A, Cabre N, Camps J, Fernández-Arroyo S, Lopez-Miranda J, Menendez J, Joven J. Nutrients in energy and one-carbon metabolism: learning from metformin users. Nutrients. 2017;9(2):121.
Diet & Lifestyle Factors: While MTHFR SNPs have received a lot of attention, there are many other factors that influence methylation. These include:
How can you tell if methylation is an issue for you? It comes down to looking at the whole picture, starting with your genes, key lab markers, and health history of symptoms.
Genetic Profiling: Through common genetic testing, such as 23andme, or more specific panels for the methylation pathway, we are now able to identify genetic variances that may have an impact on our health.
Two of the most studied SNPs have been MTHFR C677T and MTHFR A1298C.
Having MTHFR C677T heterozygous (one copy) causes a reduction in enzyme function by about 30%, whereas MTHFR C677T homozygous (two copies) results in 70 – 75% loss of enzyme activity.
However, having a genetic blueprint does not mean a particular gene or genetic mutation is expressed. Therefore, if you have been tested and found to have SNPs, it does not necessarily mean it is expressing or active. Currently, the most accurate way to assess the function of this pathway is through lab testing for functional biomarkers.
Function Lab Biomarkers for Methylation: Here are some lab levels to watch out for:
When it comes to methylation, the goal is balance. Both under-methylation (hypomethylation) and over-methylation (hypermethylation) may lead to problems, and the recent popularity in high-dose supplementation of methyl B vitamins has not been studied long term. Some research has found that over-supplementation of folate can be problematic.
Start with Food First
Nutrient deficiencies are a major cause of impaired methylation, and supporting the balance of methylation activity with food is safe and effective. Support for methylation consists of nutrient cofactors and methyl donors, including folate, B12, B6, betaine; minerals such as zinc, magnesium, iron; and sulfur proteins such as methionine.
A colorful and diverse diet high in phytonutrients with lots of folate-rich green vegetables is key!
Here are some foods that support proper methylation:
Certain nutrients can act as epigenetic regulators that impact the overall balance of methylation in the body, as is the case with anti-cancer compoundsin many herbs and spices. Some top foods that regulate methylation include cruciferous vegetables (arugula, broccoli, kale, cauliflower, cabbage), berries (blackberries, blackcurrants, blueberries, raspberries, and strawberries), mushrooms (shiitake), turmeric, and rosemary.
Support a Healthy Gut Microbiome
Gut microbes are key players in supporting methylation, producing folate, and influencing local DNA methylation patterns, modulating epigenetic activity. Eating fermented foods, taking probiotics, and incorporating prebiotic foods, such as onions, leeks, garlic, asparagus, and Jerusalem artichoke, can all be supportive for methylation.
Reduce Oxidative Stress
Stress has a huge impact on methylation, and taking steps to reduce stress in all ways will support methylation balance, such as reducing heavy metals, balancing blood sugar and insulin, addressing chronic infections, supporting sleep hygiene, and daily physical activity and exercise.
Medications that Interfere with Methylation
It’s important to be aware that medication may impede methylation activity through a variety of mechanisms, such as depleting certain B vitamins. Common drugs that inhibit methylation include antacids and PPIs, antibiotics, cholestyramine, colestipol, oral contraceptives, nitrous oxide, and metformin.
While methylation is a complex process and issue, there are clearly many ways to support this pathway using diet and lifestyle with a holistic approach tailored to the individual. Be sure to speak to your healthcare practitioner for more information about healthy methylation.
B-Complex Active Essentials
Active essentials for cellular energy production, nervous system function and cardiovascular health
Patient One B-Complex, most commonly recommended for stress, is a blend of 10 essential compounds, including the complete spectrum of B vitamins in their activated and easily absorbable forms, plus related nutrients. Our formula features Quatrefolic®, a cutting-edge active form of folate, and thiamine as Benfotiamine, which has enhanced bioavailability and easily penetrates into the inside of cells.
Thiamine (as Benfotiamine and Thiamine HCl) B1
Patient One B-Complex provides Thiamine as Benfotiamine—the fat soluble, physiologically superior form of Vitamin B-1—along with Thiamine HCl to support healthy blood sugar metabolism and protection against advanced glycation and oxidative stress.
Riboflavin (as Riboflavin-5-Phosphate) B2
Readily absorbed in the small intestine but not stored by the body, Riboflavin is an essential B vitamin. Patient One provides Riboflavin in its active form, Riboflavin-5-Phosphate, making it more easily available for use in the body. Riboflavin plays a key role in the conversion of other B vitamins and in fat and glucose metabolism. Riboflavin is also an important nutrient for healthy eyesight and skin.
Niacin (as niacinamide) B3
Niacin is an evidence-backed B vitamin that is most commonly used to support cardiovascular wellness. Research has suggested that niacin influences pathways associated with triglycerides and high-density lipoprotein (HDL) cholesterol, helping to maintain blood cholesterol levels that are already within normal range.
Pyridoxine (as Pyridoxal-5-Phosphate) B6
Pyridoxal-5-Phosphate is the metabolically active form of vitamin B-6 that has been shown to protect living lipids and proteins against glycation reactions. Aging results in the formation of advanced glycation end products (AGEs) throughout the body. By inhibiting AGE formation and working as a coenzyme in chemical reactions, pyridoxal- 5-phosphate can support healthy nerves, eyes, and cardiovascular and kidney function.
Folate (as Quatrefolic®)
Quatrefolic®, the advanced 5-MTHF (5-methyltetrahydrofolate) form of folate, bypasses metabolic steps for enhanced folate bioavailability. In vitro and in vivo studies have proven that Quatrefolic, the glucosamine salt of 5-MTHF, has greater stability, solubility and bioavailability over calcium salt forms of 5-MTHF. Folic acid is well-known for its critical role in fetal development and also supports cardiovascular health, nervous system health, normal cell growth and energy production. It plays a role in brain energy, and helps to promote peak mental sharpness and clear memory.
Vitamin B-12 (as Methylcobalamin)
Methylcobalamin is a highly bioactive form of vitamin B-12 and accounts for most B-12 circulating in plasma. Methylcobalamin B-12 supplies methyl groups for protein and DNA synthesis while supporting circulation, neurotransmitter production, and cellular energy production. B-12 also optimizes healthy homocysteine levels, promoting overall cardiovascular wellness.
Biotin has a beneficial effect on a number of structures and functions in the body. It is best known for its positive effect on hair and nails but also plays a role in supporting glucose metabolism by stimulating glucose-induced insulin secretion in pancreatic beta cells and by accelerating glucose metabolism in the liver.
Pantothenic acid (Vitamin B-5)
Pantothenic acid helps the body convert carbohydrates and fats into energy. It has been clinically proven to have a beneficial effect on lipid metabolism and adrenal immune function. It seems to positively affect triglyceride and lipoprotein levels by producing cystamine. B-5 is also considered the “anti-stress” vitamin because it supports healthy adrenal cortex function and hormone synthesis. In addition, vitamin B5 may support digestive tract health, enhancing absorption of other nutrients.
Choline is an essential nutrient that plays a vital role in maintaining the structural integrity of cell membranes. Choline is a precursor to acetylcholine, an important neurotransmitter involved in memory, muscle control and other essential functions of the body.
PABA (para-aminobenzoic acid)
PABA is a naturally occurring non-protein amino acid that works to improve the protein used in the body. It supports red blood cell formation, assists in the manufacture of folic acid in the intestines and also assists with breaking down of protein, the formation of red blood cells and maintaining intestinal flora.
Servings Per Container: 60
Take 1 vegetarian capsule as a dietary supplement, preferably with a meal, or as directed by a qualified health care professional.
Serving Size: 1 Capsule
Amount Per Serving
Thiamine ... 100mg
(Vitamin B-1) (as Thiamine HCl, Benfotiamine)
Riboflavin ... 25mg
(Vitamin B-2) (as Riboflavin-5-Phosphate)
Niacin ... 75mg
(Vitamin B-3) (as niacinamide)
Pyridoxine ... 25mg
(Vitamin B-6) (as Pyridoxal-5-Phosphate)
Folate ... 400mcg
(as Quatrefolic® [6S]-5-Methyltetrahydrofolic acid equivalent to 800 mcg of [6S]-5-Methyltetrahydrofolic acid, glucosamine salt)
Vitamin B-12 ... 400mcg
Biotin ... 300mcg
Pantothenic acid ... 100mg
(Vitamin B-5) (as D-calcium pantothenate)
Choline bitartrate ... 50mg
PABA ... 25mg
Other Ingredients: Vegetable cellulose (capsule), rice flour and l-leucine
This product contains NO milk, eggs, fish, peanuts, crustacean shellfish (lobster, crab or shrimp), soybeans, tree nuts, wheat, yeast, gluten, corn, sugar and artificial sweeteners.
This product is free of ingredients derived from genetically modified organisms (GMOs).
Quatrefolic® is a registered trademark of Gnosis S.p.A.Warnings
If you are pregnant, nursing, or taking any medications, consult your doctor before use. Discontinue use and consult doctor if any adverse reactions occur.
Supports Cognition* and Brain Health*
MagMind® (Magnesium L-Threonate, or Magtein®) is the only form of magnesium that has been shown, by promising research, to readily cross the blood-brain barrier and provide significant positive effects on cognition, memory and brain health.*
Servings Per Container: 30
Take 3 capsules per day, (2 capsules in the morning and 1 in the evening) with or without meals or as directed by your qualified healthcare professional.
Serving Size: 3 Capsules
Amount Per Serving
Magnesium ... 1, 000mg
(Elemental)(from 2,000 Magtein® Magnesium L-Threonate)
Other Ingredients: Magnesium stearate (vegetable source), cellulose and silicon dioxide. Capsule consists of bovine gelatin.
This product does not contain wheat, gluten, soybeans, dairy, egg, fish/shellfish, or peanuts/tree nuts.
Magtein® is a registered trademark of Magceutics, Inc. Multiple Patents Pending.
Note: If you have a medical condition, are pregnant, lactating, trying to conceive, under the age of 18, or taking medications, consult your healthcare professional before using this product.
Store in a cool, dry place. DO NOT refrigerate. Keep out of the reach of children.
HPA Adapt - Supporting Stress Hormone Balance via the HPA Axis with Adaptogenic Herbs*
The hypothalamic-pituitary-adrenal axis, or HPA axis, is the central part of the stress response system. It involves a complex set of interactions and feedback loops between the hypothalamus, the pituitary, and the adrenal glands. The HPA axis also plays a role in the regulation of other systems of the body, including the gastrointestinal, neurological, and immune systems. Therefore, supporting optimal HPA axis function is important to maintaining overall health and wellness.
HPA Adapt combines five powerful adaptogenic herbs to help the body better respond to both mental and physical stressors.* With key ingredients, such as Rhodiola root extract, Sensoril® Ashwagandha, and Eleuthero root extract, combined with standardized extract of Maca and Holy Basil leaf, HPA Adapt supports healthy stress hormone balance via the HPA axis.*
Servings Per Container: 30
Take 2 to 4 capsules daily on an empty stomach, or as recommended by your healthcare professional.
Serving Size: Four Veg Capsules
Amount Per Serving
Calories ... 5
Total Carbohydrate ... 1g
Rhodiola Root Extract ... 400mg
standardized to contain 3% rosavins and 1% salidroside
Ashwagandha Root and Leaf Extract ... 300mg
(Withania somnifera)(Sensoril® brand)
Eleuthero Root Extract ... 300mg
Holy Basil Leaf Extract ... 200mg
standardized to contain 2.5% triterpenoic acids including ursolic and oleanolic acids
MacaPure® brand Maca Root Extract ... 150mg
standardized to contain 0.6% macaenes and macamides
Other Ingredients: vegetable capsule (modified cellulose), ascorbyl palmitate, cellulose, silicon dioxide
This product does not contain
Do not use if pregnant. If nursing, diabetic or taking any prescription drugs, consult your healthcare professional prior to use.
Sensoril® is a trademark of Natreon, Inc. and is protected under U.S. Patents 6, 153, 198 & 7, 318, 938.
MacaPure® is a registered trademark of Naturex, Inc.
Simply the best in colostrum, ColostrumPRO with Immulox 20% enhances normal immune factors, providing dual action – in the bloodstream and in the digestive tract, to help maintain a robust intestinal lining and promote healthy intestinal flora.*
Servings Per Container: 60
Take 2 capsules, twice daily, with water or your drink of choice, on empty stomach. Triple this amount in times of environmental or physical stress.
Serving Size: 2 Capsules
Amount Per Serving:
Calories ... 10
Total Carbohydrates ... 0
Sugars ... <1g
Protein ... .5g
Bovine Colostrum ... 960mg
(Pasteurized Cow Pre-MILK)
Immunoglobulins ... 221mg
Proline-Rich Polypeptides ... 58mg
Lactoferrin ... 10mg
Other Ingredients: Medium Chain Triglycerides, Sunflower Lecithin, Gelatin (capsules).
If you have a physician-diagnosed health condition, consult your doctor before taking this product. Discontinue use if you experience adverse reactions and consult your doctor.
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.